Western Blot analysis of ACSL4 in various cell lines using a ACSL4 Recombinant Monoclonal antibody (Product # MA5-42756) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.